APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88790-PEP).
Product Note
This antibody is expected to recognise isoform c (NP_004945.4), isoform e (NP_001156768.1) and isoform f (NP_001156769.1).
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QNGKDSTAPQR, from the internal region of the protein sequence according to NP_004945.4; NP_001156768.1; NP_001156769.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
microtubule affinity regulating kinase 2 , EMK-1 , EMK1 , PAR-1 , Par-1b , Par1b
Cellular Localization
Cytoplasm , Cytoplasm, cytoskeleton , Cell projection, dendrite
Background
This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
Database
Research Area
DATA IMAGES
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GTX88790 WB Image
WB analysis of human brain (cerebellum) lysate using GTX88790 MARK2 antibody, Internal. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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