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MARK4 antibody

Anti-MARK4 antibody used in Western Blot (WB). GTX00800
Anti-MARK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00800

Cat No. GTX00800

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MARK4, corresponding to a region within the internal amino acids.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MARK4 , MARK4L , MARK4S , MARKL1 , MARKL1L , PAR1D , microtubule affinity regulating kinase 4

Cellular Localization

Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Cilium basal body,Cilium axoneme

Background

This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-MARK4 antibody used in Western Blot (WB). GTX00800

GTX00800 WB Image

WB analysis of HeLa cell lysate using GTX00800 MARK4 antibody. The lane on the left was treated with blocking peptide.

Anti-MARK4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00800

GTX00800 ICC/IF Image

ICC/IF analysis of PFA fixed MCF-7 cells using GTX00800 MARK4 antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329