APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:2000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Sheep, Bovine, Dog, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human MARK4, corresponding to a region within the internal amino acids.
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MARK4 , MARK4L , MARK4S , MARKL1 , MARKL1L , PAR1D , microtubule affinity regulating kinase 4
Cellular Localization
Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Cilium basal body,Cilium axoneme
Background
This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Database
Research Area
DATA IMAGES
|
GTX00800 ICC/IF Image
ICC/IF analysis of PFA fixed MCF-7 cells using GTX00800 MARK4 antibody. Red : Primary antibody Permeabilization : 0.1% Triton X-100 Dilution : 1:200
|
|
GTX00800 WB Image
WB analysis of HeLa cell lysate using GTX00800 MARK4 antibody. The lane on the left was treated with blocking peptide.
|
REFERENCE
There are currently no references for MARK4 antibody (GTX00800). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MARK4 antibody (GTX00800). Be the first to share your experience with this product.