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MARS antibody [N3C2], Internal

Anti-MARS antibody [N3C2], Internal used in Western Blot (WB). GTX109864
Anti-MARS antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109864
Anti-MARS antibody [N3C2], Internal used in Western Blot (WB). GTX109864

Cat. No. GTX109864

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950888

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methionyl-tRNA synthetase , CMT2U , ILFS2 , ILLD , METRS , MRS , MTRNS , SPG70

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MARS antibody [N3C2], Internal used in Western Blot (WB). GTX109864

GTX109864 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal (GTX109864) diluted at 1:1000.

Anti-MARS antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109864

GTX109864 ICC/IF Image

MARS antibody [N3C2], Internal detects MARS protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MARS protein stained by MARS antibody [N3C2], Internal (GTX109864) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-MARS antibody [N3C2], Internal used in Western Blot (WB). GTX109864

GTX109864 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal (GTX109864) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169