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MARVELD2 antibody

Anti-MARVELD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54515
Anti-MARVELD2 antibody used in Western Blot (WB). GTX54515
Anti-MARVELD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54515
Anti-MARVELD2 antibody used in Western Blot (WB). GTX54515

Cat. No. GTX54515

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μg ($399)
See all MARVELD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3 μg/mL
ICC/IF 2-4 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein derived from the C-terminal region of the human Tricellulin protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MARVEL domain containing 2 , DFNB49 , MARVD2 , MRVLDC2 , Tric

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a membrane protein found at the tight junctions between epithelial cells. The encoded protein helps establish epithelial barriers such as those in the organ of Corti, where these barriers are required for normal hearing. Defects in this gene are a cause of deafness autosomal recessive type 49 (DFNB49). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-MARVELD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54515

GTX54515 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX54515 MARVELD2 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

Anti-MARVELD2 antibody used in Western Blot (WB). GTX54515

GTX54515 WB Image

WB analysis of MDCK cell lysate using GTX54515 MARVELD2 antibody.

Anti-MARVELD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54515

GTX54515 ICC/IF Image

ICC/IF analysis of MTE7b cells using GTX54515 MARVELD2 antibody.
Panel A : No antibody control
Panel B : MARVELD2 antibody
Panel C : Occludin antibody
Panel D : Merged B and C image

Anti-MARVELD2 antibody used in Western Blot (WB). GTX54515

GTX54515 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HCT116 (Lane 1) and COLO205 (Lane 2) using GTX54515 MARVELD2 antibody.
Dilution : 1-2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399