GeneTex
United States (US)

MARVELD2 antibody

GTX54515_2029_ICC-IF_19011519_858.jpg
GTX54515_770_ICC-IF_19011519_398.jpg
GTX54515_2125_WB_19011519_589.jpg
GTX54515_1859_WB_19011519_181.jpg

Cat No. GTX54515

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS
Reactivity Human, Mouse, Rat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3 μg/mL
ICC/IF 2-4 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

64 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein derived from the C-terminal region of the human Tricellulin protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DFNB49,MARVD2,MRVLDC2,Tric

Cellular Localization

Cell junction,Cell membrane,Tight junction

Background

The protein encoded by this gene is a membrane protein found at the tight junctions between epithelial cells. The encoded protein helps establish epithelial barriers such as those in the organ of Corti, where these barriers are required for normal hearing. Defects in this gene are a cause of deafness autosomal recessive type 49 (DFNB49). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

DATA IMAGES
GTX54515_2029_ICC-IF_19011519_858.jpg

GTX54515 ICC/IF Image

ICC/IF analysis of MTE7b cells using GTX54515 MARVELD2 antibody.
Panel A : No antibody control
Panel B : MARVELD2 antibody
Panel C : Occludin antibody
Panel D : Merged B and C image

GTX54515_770_ICC-IF_19011519_398.jpg

GTX54515 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX54515 MARVELD2 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 µg/ml in 0.1% BSA incubated for 3 hours at room temperature

GTX54515_2125_WB_19011519_589.jpg

GTX54515 WB Image

WB analysis of MDCK cell lysate using GTX54515 MARVELD2 antibody.

GTX54515_1859_WB_19011519_181.jpg

GTX54515 WB Image

WB analysis of whole cell extracts (30 µg lysate) of HCT116 (Lane 1) and COLO205 (Lane 2) using GTX54515 MARVELD2 antibody.
Dilution : 1-2 µg/ml

Package List Price ($)
$ 329