APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500~1:1000 |
Not tested in other applications.
Calculated MW
Product Note
MASP1 (cleaved Arg448) antibody detects endogenous levels of fragment of activated MASP1 resulting from cleavage adjacent to Arg448.
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human MASP1 (399-448).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
3MC1 , CRARF , CRARF1 , MAP1 , MASP , MASP1 , MASP3 , MAp44 , PRSS5 , RaRF , mannan binding lectin serine peptidase 1 , mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
Cellular Localization
Secreted
Background
This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
Database
Research Area
DATA IMAGES
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GTX86929 WB Image
WB analysis of A549 cells treated with etoposide 25uM (24h) lysate using GTX86929 MASP1 (cleaved Arg448) antibody. The lane on the right is blocked with the synthesized peptide.
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REFERENCE
There are currently no references for MASP1 (cleaved Arg448) antibody (GTX86929). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MASP1 (cleaved Arg448) antibody (GTX86929). Be the first to share your experience with this product.
