GeneTex
United States (US)

MASP1 (cleaved Arg448) antibody

MASP1-cleaved-Arg448-antibody-GTX86929-WB-1_18121411_874.jpg

Cat No. GTX86929

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

47 kDa. ( Note )

Specificity/Sensitivity

MASP1 (heavy chain,Cleaved-Arg448) Antibody detects endogenous levels of fragment of activated MASP1 resulting from cleavage adjacent to Arg448.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MASP1.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mannan Binding Lectin Serine Peptidase 1,3Mc1,Crarf,Crarf1,Map1,Masp,Masp3,Map44,Prss5,Rarf,Masp1

Background

Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.

Database

Research Area

DATA IMAGES
MASP1-cleaved-Arg448-antibody-GTX86929-WB-1_18121411_874.jpg

GTX86929 WB Image

Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using MASP1 (heavy chain,Cleaved-Arg448) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339