Wild-type (WT) and MASPIN knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MASPIN antibody [N3C3] (GTX101292) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using MASPIN(GTX101292) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
10% SDS PAGE
GTX101292 diluted at 1:1000