GeneTex
United States (US)

MASPIN antibody [N3C3]

GTX101292_39721_IHC_19011506_691.jpg
GTX101292_WB_19011506_663.jpg

Cat No. GTX101292

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

A431 , HeLaS3 , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MASPIN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PI5 antibody, maspin antibody, SERPINB5 antibody, peptidase inhibitor 5 antibody, "serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 antibody", serpin B5 antibody, PI-5 antibody, protease inhibitor 5 (maspin) antibody, "serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody"

Cellular Localization

Secreted , extracellular space

Background

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
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Database

Research Area

DATA IMAGES
GTX101292_39721_IHC_19011506_691.jpg

GTX101292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using MASPIN(GTX101292) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101292_WB_19011506_663.jpg

GTX101292 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
10% SDS PAGE
GTX101292 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf