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MASPIN antibody

Anti-MASPIN antibody used in Western Blot (WB). GTX100471
Anti-MASPIN antibody used in Western Blot (WB). GTX100471
Anti-MASPIN antibody used in Western Blot (WB). GTX100471
Anti-MASPIN antibody used in IHC (Paraffin sections) (IHC-P). GTX100471

Cat No. GTX100471

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLaS3 , NIH-3T3

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MASPIN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family B member 5 , PI5 , maspin

Cellular Localization

Secreted , extracellular space

Background

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Database

Research Area

DATA IMAGES

Anti-MASPIN antibody used in Western Blot (WB). GTX100471

GTX100471 WB Image

Wild-type (WT) and MASPIN knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MASPIN antibody (GTX100471) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MASPIN antibody used in Western Blot (WB). GTX100471

GTX100471 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX100471 diluted at 1:1000

Anti-MASPIN antibody used in Western Blot (WB). GTX100471

GTX100471 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX100471 diluted at 1:1000

Anti-MASPIN antibody used in IHC (Paraffin sections) (IHC-P). GTX100471

GTX100471 IHC-P Image

MASPIN antibody detects SERPINB5 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MASPIN antibody (GTX100471) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169