Wild-type (WT) and MASPIN knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MASPIN antibody (GTX100471) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MASPIN antibody detects SERPINB5 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MASPIN antibody (GTX100471) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX100471 diluted at 1:1000

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX100471 diluted at 1:1000