Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAST1 antibody [N2N3] (GTX107508) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (50 μg of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX107508 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.