GeneTex

  • Country / Location Selection

United States (US)

MAT1A + MAT2A antibody [N1C2]

Orthogonal
Anti-MAT1A + MAT2A antibody [N1C2] used in Western Blot (WB). GTX112535
Anti-MAT1A + MAT2A antibody [N1C2] used in Western Blot (WB). GTX112535
Anti-MAT1A + MAT2A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX112535
Anti-MAT1A + MAT2A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112535
Anti-MAT1A + MAT2A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX112535
Anti-MAT1A + MAT2A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX112535

Cat. No. GTX112535

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MAT1A + MAT2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAT2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177481

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methionine adenosyltransferase 2A , MATA2 , MATII , SAMS2

Background

Catalyzes the formation of S-adenosylmethionine from methionine and ATP.

Database

Research Area

DATA IMAGES

Anti-MAT1A + MAT2A antibody [N1C2] used in Western Blot (WB). GTX112535

GTX112535 WB Image

Various whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody (GTX112535) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAT1A + MAT2A antibody [N1C2] used in Western Blot (WB). GTX112535

GTX112535 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody (GTX112535) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MAT1A + MAT2A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX112535

GTX112535 IHC-P Image

MAT1A + MAT2A antibody [N1C2] detects MAT2A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MAT2A stained by MAT1A + MAT2A antibody [N1C2] (GTX112535) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAT1A + MAT2A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112535

GTX112535 ICC/IF Image

MAT1A + MAT2A antibody [N1C2] detects MAT2A protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAT2A stained by MAT1A + MAT2A antibody [N1C2] (GTX112535) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAT1A + MAT2A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX112535

GTX112535 IHC-P Image

MAT1A + MAT2A antibody [N1C2] detects MAT2A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MAT2A stained by MAT1A + MAT2A antibody [N1C2] (GTX112535) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAT1A + MAT2A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX112535

GTX112535 IHC-P Image

MAT1A + MAT2A antibody [N1C2] detects MAT2A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
MAT2A stained by MAT1A + MAT2A antibody [N1C2] (GTX112535) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Pei-Yi Chu et al. Int J Mol Sci 2021; 22 (10) MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients.
Sviripa VM et al. Medchemcomm 2018; 9 (1) : 87-99 Phenylethynyl-substituted Heterocycles Inhibit Cyclin D1 and Induce the Expression of Cyclin-dependent Kinase Inhibitor p21Wif1/Cip1 in Colorectal Cancer Cells.
Submit a Reference

REVIEW

There are currently no reviews for MAT1A + MAT2A antibody [N1C2] (GTX112535). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169