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MAT2A antibody [AT3A2]

Orthogonal
Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027
Anti-MAT2A antibody [AT3A2] used in IHC (Paraffin sections) (IHC-P). GTX50027
Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027
Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027
Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027

Cat. No. GTX50027

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT3A2

Isotype

 IgG2b

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MAT2A products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

44 kDa. ( Note )

Product Note

Based on the blast result, this antibody may cross-react with human MAT1A.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MAT2A (1-395aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

RRID

AB_11161922

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methionine adenosyltransferase 2A , MATA2 , MATII , SAMS2

Background

The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027

GTX50027 WB Image

Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-MAT2A antibody [AT3A2] used in IHC (Paraffin sections) (IHC-P). GTX50027

GTX50027 IHC-P Image

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50, GTX50027) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027

GTX50027 WB Image

Orthogonal

The data was published in the 2022 in Signal Transduct Target Ther. PMID: 35729157

Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027

GTX50027 WB Image

Orthogonal

The data was published in the 2022 in Signal Transduct Target Ther. PMID: 35729157

Anti-MAT2A antibody [AT3A2] used in Western Blot (WB). GTX50027

GTX50027 WB Image

The data was published in the 2022 in Signal Transduct Target Ther. PMID: 35729157

REFERENCE

Application Reference

Chu PY et al. Int J Mol Sci 2023; 24 (10) Translocation of Methionine Adenosyl Transferase MAT2A and Its Prognostic Relevance for Liver Hepatocellular Carcinoma.
Jin-Tao Li et al. Signal Transduct Target Ther 2022; 7 (1) : 192 Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA.
Application : WB
Reactivity : Human
Pei-Yi Chu et al. Int J Mol Sci 2021; 22 (10) MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients.
Application : WB, IHC-P
Reactivity : Human
Wang Z et al. Nat Med 2019; 25 (5) : 825-837 Methionine is a metabolic dependency of tumor-initiating cells.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399