Wild-type (WT) and MAT2B knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT2B antibody [N3C3] (GTX115863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MAT2B(GTX115863) antibody at 1:200 dilution.