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MAX antibody

KO/KD IP/MS
Anti-MAX antibody used in Immunoprecipitation (IP). GTX113492
Anti-MAX antibody used in Western Blot (WB). GTX113492
Anti-MAX antibody used in Western Blot (WB). GTX113492
Anti-MAX antibody used in Western Blot (WB). GTX113492

Cat. No. GTX113492

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all MAX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Product Note

IP/MS validation was supported by references (PMID:30377412)

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163945

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MYC associated factor X , bHLHd4

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAX antibody used in Immunoprecipitation (IP). GTX113492

GTX113492 IP Image

MAX antibody immunoprecipitates MAX protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of MAX protein by 2 μg of MAX antibody (GTX113492) 15% SDS-PAGE The immunoprecipitated MAX protein was detected by MAX antibody (GTX113492) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-MAX antibody used in Western Blot (WB). GTX113492

GTX113492 WB Image

KO/KD

Wild-type (WT) and MAX knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody (GTX113492) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAX antibody used in Western Blot (WB). GTX113492

GTX113492 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody (GTX113492) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MAX antibody used in Western Blot (WB). GTX113492

GTX113492 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody (GTX113492) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chang TM et al. Cancer Sci 2020; (Epub) c-Myc promotes lymphatic metastasis of pancreatic neuroendocrine tumor through VEGFC upregulation.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169