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MBD1 antibody [100B272.1]

Anti-MBD1 antibody [100B272.1] used in Western Blot (WB). GTX76673

Cat. No. GTX76673

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 100B272.1

Isotype

 IgG1

Application

 WB, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all MBD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 4 μg/ml
IP Assay dependent
ChIP assay 1:10 - 1:500
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Product Note

There are many isoforms of human MBD1 ranging from 503 (~ 55kD) to 655 (~ 71kD) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this antibody is suitable for testing a specific isoform of interest.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_510217

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyl-CpG binding domain protein 1 , CXXC3 , PCM1 , RFT

Cellular Localization

Nucleus,Nucleus speckle

Database

Research Area

DATA IMAGES

Anti-MBD1 antibody [100B272.1] used in Western Blot (WB). GTX76673

GTX76673 WB Image

WB analysis of HeLa cell lysate using GTX76673 MBD1 antibody [100B272.1].
Lane 1 : 2 ug/ml primary antibody
Lane 2 : 1 ug/ml primary antibody

REFERENCE

Clone Reference

Reese BE et al. Mol Cell Biol 2003; 23 (9):3226-36 The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399