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MBD1 antibody [100B272.1]

Anti-MBD1 antibody [100B272.1] used in Western Blot (WB). GTX76673

Cat No. GTX76673

Host

Mouse

Clonality

Monoclonal

Clone Name

100B272.1

Isotype

IgG1

Application

WB, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 - 4 μg/ml
IP Assay dependent
ChIP assay 1:10 - 1:500
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Specificity/Sensitivity

There are many isoforms of human MBD1 ranging from 503 (~ 55kD) to 655 (~ 71kD) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this antibody is suitable for testing a specific isoform of interest.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

methyl-CpG binding domain protein 1 , CXXC3 , PCM1 , RFT

Cellular Localization

Nucleus,Nucleus speckle

Database

Research Area

DATA IMAGES

Anti-MBD1 antibody [100B272.1] used in Western Blot (WB). GTX76673

GTX76673 WB Image

WB analysis of HeLa cell lysate using GTX76673 MBD1 antibody [100B272.1].
Lane 1 : 2 ug/ml primary antibody
Lane 2 : 1 ug/ml primary antibody

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339