MBD1 antibody - ChIP grade

Cat. No. GTX60359

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA, ChIP assay
Reactivity	Human, Mouse
Package	50 μg ($429)

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Datasheet

View product citations for antibody GTX60359 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ELISA	1:1,000
ChIP assay	1-5 μg

Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human MBD1 (Methyl-CpG-binding domain protein 1), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyl-CpG binding domain protein 1 , CXXC3 , PCM1 , RFT

Cellular Localization

Nucleus , Nucleus speckle

Background

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]

Database

Gene ID: 4152 MBD1
UniProt: Q9UIS9 MBD1

Research Area

DATA IMAGES

GTX60359 WB Image

WB analysis of nuclear extracts (40 μg) from HeLa cells using GTX60359 MBD1 antibody - ChIP grade.
Dilution : 1:500

GTX60359 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ U2OS cells using GTX60359 MBD1 antibody - ChIP grade. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

GTX60359 ELISA Image

ELISA analysis of peptides used for immunization using GTX60359 MBD1 antibody - ChIP grade.

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Package

List Price ($)

50 μg

$ 429

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