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MBD2 antibody, Internal

KO/KD
Anti-MBD2 antibody, Internal used in Western Blot (WB). GTX82965
Anti-MBD2 antibody, Internal used in Western Blot (WB). GTX82965
Anti-MBD2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX82965

Cat. No. GTX82965

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all MBD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HepG2 , mouse ES cells

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human MBD2

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_1081276

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyl-CpG binding domain protein 2 , DMTase , NY-CO-41

Cellular Localization

Nucleus

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-MBD2 antibody, Internal used in Western Blot (WB). GTX82965

GTX82965 WB Image

WB analysis of HepG2 cells using GTX82965 MBD2 antibody at 0.625μg/ml.
Lane A : marker
Lane B : HepG2 cells

Anti-MBD2 antibody, Internal used in Western Blot (WB). GTX82965

GTX82965 WB Image

KO/KD

WB analysis of mouse ES cells using GTX82965 MBD2 antibody at 1:1000.Loading amount : 15ug.
Lane 1 : MBD2 KO mouse ES cell lysate
Lane 2 : Wild type mouse ES cell lysate

Anti-MBD2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX82965

GTX82965 IHC-P Image

IHC-P analysis of human heart tissue using GTX82965 MBD2 antibody at 1:100. Left to right:DAPI, MBD2, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.

REFERENCE

Application Reference

Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399