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MBD3 antibody [106B691]

Anti-MBD3 antibody [106B691] used in IHC (Paraffin sections) (IHC-P). GTX16309

Cat No. GTX16309

Host

Mouse

Clonality

Monoclonal

Clone Name

106B691

Isotype

IgG1

Application

WB, IHC-P

Reactivity

Human, Bovine, Dog, Chicken, Pig, Bat, Gibbon, Gorilla, Horse, Orangutan, Xenopus
Package
50 μg ($659)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 5 μg/mL
IHC-P 5 μg/mL
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this may detect human, mouse, and rat MBD3 (100%), and human and mouse MBD2 (93%).

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MBD3 monoclonal antibody was raised against amino acids 215 - 230 of MBD3 (Human).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

methyl-CpG binding domain protein 3

Cellular Localization

Nucleus

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MBD3 antibody [106B691] used in IHC (Paraffin sections) (IHC-P). GTX16309

GTX16309 IHC-P Image

IHC-P analysis of human kidney tissue using GTX16309 MBD3 antibody [106B691].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf