APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 nuclear
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.51 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MBD3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10621145
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
methyl-CpG binding domain protein 3
Cellular Localization
Nucleus
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX116204 WB Image
Western blot experiment using MBD3 Antibody (GTX116204) at 1:90000 dilution to detect 100ng of 27kDa recombinant MBD3 protein antigen.
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GTX116204 WB Image
Sample (20 ug ) A: A431 nucleus 12% SDS PAGE GTX116204 diluted at 1:1000
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REFERENCE
There are currently no references for MBD3 antibody [N1C3] (GTX116204). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MBD3 antibody [N1C3] (GTX116204). Be the first to share your experience with this product.