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MC1 Receptor antibody [HL1471]

Recombinant antibody KO/KD Orthogonal
Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in IHC (Frozen sections) (IHC-Fr). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945
Anti-MC1 Receptor antibody [HL1471] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636945

Cat. No. GTX636945

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1471

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MC1 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

This antibody was raised against human MC1 Receptor Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human MC1 Receptor. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanocortin 1 receptor , CMM5 , MSH-R , SHEP2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945

GTX636945 WB Image

KO/KD Orthogonal

Non-transfected (–) and transfected (+) A375 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945

GTX636945 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945

GTX636945 IHC-P Image

MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded A375 xenograft .
MC1 Receptor stained by MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945

GTX636945 WB Image

Various extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945

GTX636945 IHC-P Image

MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MC1 Receptor stained by MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945

GTX636945 IHC-P Image

MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
MC1 Receptor stained by MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945

GTX636945 IHC-P Image

MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
MC1 Receptor stained by MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MC1 Receptor antibody [HL1471] used in IHC (Paraffin sections) (IHC-P). GTX636945

GTX636945 IHC-P Image

MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
MC1 Receptor stained by MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MC1 Receptor antibody [HL1471] used in IHC (Frozen sections) (IHC-Fr). GTX636945

GTX636945 IHC-Fr Image

MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MC1 Receptor antibody [HL1471] used in Western Blot (WB). GTX636945

GTX636945 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MC1 Receptor antibody [HL1471] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636945

GTX636945 ICC/IF Image

MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in ice-cold MeOH for 5 min.
Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] (GTX636945) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations