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MCAK antibody [N1N3]

Anti-MCAK antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103013
Anti-MCAK antibody [N1N3] used in Western Blot (WB). GTX103013

Cat No. GTX103013

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

kinesin family member 2C , CT139 , KNSL6 , MCAK

Cellular Localization

Cytoplasm , cytoskeleton , Nucleus , Centromere

Background

The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MCAK antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103013

GTX103013 ICC/IF Image

MCAK antibody [N1N3] detects MCAK protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCAK protein stained by MCAK antibody [N1N3] (GTX103013) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-MCAK antibody [N1N3] used in Western Blot (WB). GTX103013

GTX103013 WB Image

Sample (30 ug of whole cell lysate)
A: K562
7.5% SDS PAGE
GTX103013 diluted at 1:1000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169