APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HepG2 , mouse liver
Predict Reactivity
Rat, Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MCCC2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
MCCB antibody, MCCC2 antibody, 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit antibody, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta antibody, biotin carboxylase antibody, MCCase subunit beta antibody, 3-methylcrotonyl-CoA carboxylase 2 antibody, "methylcrotonoyl-CoA carboxylase beta chain, mitochondrial antibody", methylcrotonoyl-Coenzyme A carboxylase 2 (beta) antibody, non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase antibody, methylcrotonoyl-CoA carboxylase 2 (beta) antibody
Cellular Localization
Mitochondrion matrix
Background
This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102674 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MCCC2(GTX102674) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102674 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX102674 diluted at 1:1000
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GTX102674 IHC-P Image
MCCC2 antibody [N1C1] detects MCCC2 protein at mitochondria in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
MCCC2 antibody [N1C1] (GTX102674) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102674 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX102674 diluted at 1:1000
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