GeneTex
United States (US)

MCCC2 antibody [N1C1]

GTX102674_40114_IHC_19011506_959.jpg
GTX102674_40114_WB_M_liver_19011506_765.jpg
GTX102674_40114_20150529_IHC_M_19011506_370.jpg
GTX102674_WB_19011506_181.jpg

Cat No. GTX102674

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HepG2 , mouse liver

Predict Reactivity

Rat, Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCCC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MCCB antibody, MCCC2 antibody, 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit antibody, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta antibody, biotin carboxylase antibody, MCCase subunit beta antibody, 3-methylcrotonyl-CoA carboxylase 2 antibody, "methylcrotonoyl-CoA carboxylase beta chain, mitochondrial antibody", methylcrotonoyl-Coenzyme A carboxylase 2 (beta) antibody, non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase antibody, methylcrotonoyl-CoA carboxylase 2 (beta) antibody

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102674_40114_IHC_19011506_959.jpg

GTX102674 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MCCC2(GTX102674) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102674_40114_WB_M_liver_19011506_765.jpg

GTX102674 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX102674 diluted at 1:1000

GTX102674_40114_20150529_IHC_M_19011506_370.jpg

GTX102674 IHC-P Image

MCCC2 antibody [N1C1] detects MCCC2 protein at mitochondria in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
MCCC2 antibody [N1C1] (GTX102674) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102674_WB_19011506_181.jpg

GTX102674 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX102674 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169