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MCCC2 antibody [N1C1]

Anti-MCCC2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102674
Anti-MCCC2 antibody [N1C1] used in Western Blot (WB). GTX102674
Anti-MCCC2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102674
Anti-MCCC2 antibody [N1C1] used in Western Blot (WB). GTX102674

Cat No. GTX102674

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
See all MCCC2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HepG2 , mouse liver

Predict Reactivity

Rat, Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCCC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

methylcrotonoyl-CoA carboxylase 2 , MCCB

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-MCCC2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102674

GTX102674 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MCCC2(GTX102674) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-MCCC2 antibody [N1C1] used in Western Blot (WB). GTX102674

GTX102674 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX102674 diluted at 1:1000

Anti-MCCC2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102674

GTX102674 IHC-P Image

MCCC2 antibody [N1C1] detects MCCC2 protein at mitochondria in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
MCCC2 antibody [N1C1] (GTX102674) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anti-MCCC2 antibody [N1C1] used in Western Blot (WB). GTX102674

GTX102674 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX102674 diluted at 1:1000
REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169