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MCD antibody [HL2738]

Recombinant antibody Comparison Orthogonal
Anti-MCD antibody [HL2738] used in Western Blot (WB). GTX639567
Anti-MCD antibody [HL2738] used in Western Blot (WB). GTX639567
Anti-MCD antibody [HL2738] used in IHC (Paraffin sections) (IHC-P). GTX639567

Cat. No. GTX639567

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2738

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MCD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human MCD.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malonyl-CoA decarboxylase , MCD

Cellular Localization

Cytoplasm,Mitochondrion matrix

Background

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MCD antibody [HL2738] used in Western Blot (WB). GTX639567

GTX639567 WB Image

Comparison

Various tissue extracts (5 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with MCD antibody [HL2738] (GTX639567) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-MCD antibody [HL2738] used in Western Blot (WB). GTX639567

GTX639567 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCD antibody [HL2738] (GTX639567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MCD antibody [HL2738] used in IHC (Paraffin sections) (IHC-P). GTX639567

GTX639567 IHC-P Image

MCD antibody [HL2738] detects MCD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
MCD stained by MCD antibody [HL2738] (GTX639567) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations