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MCD antibody [N2C1], Internal

Orthogonal
Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037
Anti-MCD antibody [N2C1], Internal used in Western Blot (WB). GTX110037
Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037
Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037
Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037
Anti-MCD antibody [N2C1], Internal used in Western Blot (WB). GTX110037
Anti-MCD antibody [N2C1], Internal used in Western Blot (WB). GTX110037

Cat. No. GTX110037

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MCD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

C8D30

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950924

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malonyl-CoA decarboxylase , MCD

Cellular Localization

Mitochondrion , Cytoplasm , Peroxisome

Background

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037

GTX110037 IHC-P Image

MCD antibody [N2C1], Internal detects MCD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
MCD stained by MCD antibody [N2C1], Internal (GTX110037) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MCD antibody [N2C1], Internal used in Western Blot (WB). GTX110037

GTX110037 WB Image

Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037

GTX110037 IHC-P Image

MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037

GTX110037 IHC-P Image

MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCD antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110037

GTX110037 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using MCD(GTX110037) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCD antibody [N2C1], Internal used in Western Blot (WB). GTX110037

GTX110037 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MCD antibody [N2C1], Internal used in Western Blot (WB). GTX110037

GTX110037 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169