Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

MCD antibody [N2C1], Internal detects MCD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
MCD stained by MCD antibody [N2C1], Internal (GTX110037) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using MCD(GTX110037) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min