MCD antibody [N2C1], Internal

Cat. No. GTX110037

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all MCD products

Datasheet
Document

View product citations for antibody GTX110037 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

C8D30

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950924

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

malonyl-CoA decarboxylase , MCD

Cellular Localization

Mitochondrion , Cytoplasm , Peroxisome

Background

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq]

Database

Gene ID: 23417 MLYCD
UniProt: O95822 MLYCD

Research Area

DATA IMAGES

GTX110037 IHC-P Image

MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110037 ICC/IF Image

MCD antibody [N2C1], Internal detects MCD protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCD stained by MCD antibody [N2C1], Internal (GTX110037) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX110037 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110037 IHC-P Image

MCD antibody [N2C1], Internal detects MCD protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MCD antibody [N2C1], Internal (GTX110037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110037 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal (GTX110037) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110037 WB Image

MCD antibody detects MCD protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
7.5% SDS-PAGE
MCD antibody (GTX110037) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110037 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using MCD(GTX110037) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110037 IHC-P Image

MCD antibody [N2C1], Internal detects MCD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
MCD stained by MCD antibody [N2C1], Internal (GTX110037) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

Currently in production

MCD antibody [N2C1], Internal

Cat. No. GTX110037

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX110037 IHC-P Image

GTX110037 ICC/IF Image

GTX110037 WB Image

GTX110037 IHC-P Image

GTX110037 WB Image

GTX110037 WB Image

GTX110037 IHC-P Image

GTX110037 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.