MCL1 antibody [10F6]

Cat. No. GTX84140

Host	Mouse
Clonality	Monoclonal
Clone Name	10F6
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($519)

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Datasheet
Document

View product citations for antibody GTX84140 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MCL1 apoptosis regulator, BCL2 family member , BCL2L3 , EAT , MCL1-ES , MCL1L , MCL1S , Mcl-1 , TM , bcl2-L-3 , mcl1/EAT

Cellular Localization

Membrane,Cytoplasm,Mitochondrion,nucleoplasm

Background

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Database

Gene ID: 4170 MCL1
UniProt: Q07820 MCL1

Research Area

DATA IMAGES

GTX84140 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84140 MCL1 antibody [10F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84140 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84140 MCL1 antibody [10F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84140 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with MCL1 plasmid using GTX84140 MCL1 antibody [10F6].

GTX84140 WB Image

WB analysis of HEK293T cells transfected with MCL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84140 MCL1 antibody [10F6].
Loading : 5 ug per lane

GTX84140 IHC-P Image

IHC-P analysis of breast tissue using GTX84140 MCL1 antibody [10F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84140 FACS Image

FACS analysis of HEK293T cells transfected with either MCL1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84140 MCL1 antibody [10F6].

GTX84140 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX84140 MCL1 antibody [10F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84140 WB Image

WB analysis of Jurkat cell lysate using GTX84140 MCL1 antibody [10F6].
Loading : 10 ug per lane
Dilution : 1:200

GTX84140 IHC-P Image

IHC-P analysis of kidney tissue using GTX84140 MCL1 antibody [10F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84140 IHC-P Image

IHC-P analysis of prostate tissue using GTX84140 MCL1 antibody [10F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

MCL1 antibody [10F6]

Cat. No. GTX84140

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84140 IHC-P Image

GTX84140 IHC-P Image

GTX84140 ICC/IF Image

GTX84140 WB Image

GTX84140 IHC-P Image

GTX84140 FACS Image

GTX84140 IHC-P Image

GTX84140 WB Image

GTX84140 IHC-P Image

GTX84140 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.