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MCL1 antibody [2E11]

Overexpression
Anti-MCL1 antibody [2E11] used in Flow cytometry (FACS). GTX84135
Anti-MCL1 antibody [2E11] used in Western Blot (WB). GTX84135
Anti-MCL1 antibody [2E11] used in Western Blot (WB). GTX84135
Anti-MCL1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84135
Anti-MCL1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84135
Anti-MCL1 antibody [2E11] used in Flow cytometry (FACS). GTX84135
Anti-MCL1 antibody [2E11] used in Flow cytometry (FACS). GTX84135

Cat. No. GTX84135

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2E11

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all MCL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MCL1 apoptosis regulator, BCL2 family member , BCL2L3 , EAT , MCL1-ES , MCL1L , MCL1S , Mcl-1 , TM , bcl2-L-3 , mcl1/EAT

Cellular Localization

Membrane,Cytoplasm,Mitochondrion,nucleoplasm

Background

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Database

Research Area

DATA IMAGES

Anti-MCL1 antibody [2E11] used in Flow cytometry (FACS). GTX84135

GTX84135 FACS Image

FACS analysis of HeLa cells using GTX84135 MCL1 antibody [2E11].
Red : Primary antibody
Blue : Negative control antibody

Anti-MCL1 antibody [2E11] used in Western Blot (WB). GTX84135

GTX84135 WB Image

Overexpression

WB analysis of HEK293T cells transfected with MCL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84135 MCL1 antibody [2E11].
Loading : 5 ug per lane

Anti-MCL1 antibody [2E11] used in Western Blot (WB). GTX84135

GTX84135 WB Image

WB analysis of various cell lines using GTX84135 MCL1 antibody [2E11].
Loading : 10 ug per lane
Dilution : 1:200

Anti-MCL1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84135

GTX84135 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with MCL1 plasmid using GTX84135 MCL1 antibody [2E11].

Anti-MCL1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84135

GTX84135 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84135 MCL1 antibody [2E11].

Anti-MCL1 antibody [2E11] used in Flow cytometry (FACS). GTX84135

GTX84135 FACS Image

FACS analysis of Jurkat cells using GTX84135 MCL1 antibody [2E11].
Red : Primary antibody
Blue : Negative control antibody

Anti-MCL1 antibody [2E11] used in Flow cytometry (FACS). GTX84135

GTX84135 FACS Image

FACS analysis of HEK293T cells using GTX84135 MCL1 antibody [2E11].
Black : Cell only
Blue : Primary antibody
Red : Isotype control antibody

REFERENCE

Application Reference

Shi K et al. Cell Death Differ 2023; 30 (1) : 195-207 Transient targeting of BIM-dependent adaptive MCL1 preservation enhances tumor response to molecular therapeutics in non-small cell lung cancer.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519