MCL1 antibody

Cat. No. GTX102026

Reference 10

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all MCL1 products

View product citations for antibody GTX102026 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Raji , NCI-H929

Predict Reactivity

Rat, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950896

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MCL1 apoptosis regulator, BCL2 family member , BCL2L3 , EAT , MCL1-ES , MCL1L , MCL1S , Mcl-1 , TM , bcl2-L-3 , mcl1/EAT

Cellular Localization

Membrane; Single-pass membrane protein (Potential) , Cytoplasm , Mitochondrion , Nucleus , nucleoplasm

Background

The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. [provided by RefSeq]

Database

Gene ID: 4170 MCL1
UniProt: Q07820 MCL1

Research Area

DATA IMAGES

GTX102026 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody (GTX102026) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102026 WB Image

MCL1 antibody detects MCL1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody (GTX102026) diluted at 1:1000.

GTX102026 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MCL1(GTX102026) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102026 ICC/IF Image

MCL1 antibody detects MCL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCL1 protein stained by MCL1 antibody (GTX102026) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX102026 IHC-P Image

MCL1 antibody detects MCL1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
MCL1 antibody (GTX102026) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min