GeneTex
United States (US)

MCL1 antibody

GTX102026_39974_IHC_19011506_111.jpg
GTX102026_42242_20151102_IHC-P_M_19011506_471.jpg
GTX102026_42242_20150924_WB_19011506_769.jpg
GTX102026_WB_19011506_897.jpg
GTX102026_39974_20150410_IFA_19011506_522.jpg

Cat No. GTX102026

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Raji , NCI-H929

Predict Reactivity

Rat, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mcl1, Bcl2 Family Apoptosis Regulator,Bcl2L3,Eat,Mcl1-Es,Mcl1L,Mcl1S,Mcl-1,Tm,Bcl2-L-3,Mcl1/Eat,Mcl1

Cellular Localization

Membrane; Single-pass membrane protein (Potential) , Cytoplasm , Mitochondrion , Nucleus , nucleoplasm

Background

The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102026_39974_IHC_19011506_111.jpg

GTX102026 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MCL1(GTX102026) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102026_42242_20151102_IHC-P_M_19011506_471.jpg

GTX102026 IHC-P Image

MCL1 antibody detects MCL1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
MCL1 antibody (GTX102026) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102026_42242_20150924_WB_19011506_769.jpg

GTX102026 WB Image

MCL1 antibody detects MCL1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCL1 antibody (GTX102026) diluted at 1:1000.

GTX102026_WB_19011506_897.jpg

GTX102026 WB Image

Sample (30μg whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX102026 diluted at 1:1000

GTX102026_39974_20150410_IFA_19011506_522.jpg

GTX102026 ICC/IF Image

MCL1 antibody detects MCL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCL1 protein stained by MCL1 antibody (GTX102026) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169