APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Raji Cell Slide(GTX25538) , Raji Cell Lysate(GTX27908)
Product Note
This Mcl-1 antibody detects isoforms Mcl-1L and Mcl-1ES.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Mcl-1.The immunogen is located within amino acids 220 - 270 of Mcl-1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MCL1 apoptosis regulator, BCL2 family member , BCL2L3 , EAT , MCL1-ES , MCL1L , MCL1S , Mcl-1 , TM , bcl2-L-3 , mcl1/EAT
Cellular Localization
Membrane,Cytoplasm,Mitochondrion,nucleoplasm
Background
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
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GTX31708 WB Image
WB analysis of Raji cell lysate using GTX31708 MCL1 antibody.
Working concentration : 0.5 μg/ml
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GTX31708 ICC/IF Image
ICC/IF analysis of Raji cells using GTX31708 MCL1 antibody.
Working concentration : 10 μg/ml
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GTX31708 ICC/IF Image
ICC/IF analysis of Raji cells using GTX31708 MCL1 antibody.
Working concentration : 2 μg/ml
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REFERENCE
There are currently no references for MCL1 antibody (GTX31708). Be the first to share your publications with this product.
REVIEW
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