GeneTex
United States (US)

MCL1 antibody

GTX31709_294_ICC-IF_20180221_18121410_407.jpg
GTX31709_295_ICC-IF_20180221_18121410_336.jpg
GTX31709_2873_WB_20180221_18121410_403.jpg

Cat No. GTX31709

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Raji Cell Slide(GTX25538) , Raji Cell Lysate(GTX27908)

Specificity/Sensitivity

Detects isoforms Mcl-1L and Mcl-1S
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Mcl-1.The immunogen is located within amino acids 20 - 70 of Mcl-1.

Purification

Purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mcl1, Bcl2 Family Apoptosis Regulator,Bcl2L3,Eat,Mcl1-Es,Mcl1L,Mcl1S,Mcl-1,Tm,Bcl2-L-3,Mcl1/Eat,Mcl1

Background

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES
GTX31709_294_ICC-IF_20180221_18121410_407.jpg

GTX31709 ICC/IF Image

ICC/IF analysis of Raji cells using GTX31709 MCL1 antibody.
Working concentration : 10 μg/ml

GTX31709_295_ICC-IF_20180221_18121410_336.jpg

GTX31709 ICC/IF Image

ICC/IF analysis of Raji cells using GTX31709 MCL1 antibody.
Working concentration : 20 μg/ml

GTX31709_2873_WB_20180221_18121410_403.jpg

GTX31709 WB Image

WB analysis of Raji cell lysate using GTX31709 MCL1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Package List Price ($)
$ 329