APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide of human MCL1
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MCL1 apoptosis regulator, BCL2 family member , BCL2L3 , EAT , MCL1-ES , MCL1L , MCL1S , Mcl-1 , TM , bcl2-L-3 , mcl1/EAT
Cellular Localization
Membrane,Cytoplasm,Mitochondrion,nucleoplasm
Background
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
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GTX55698 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX55698 MCL1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX55698 WB Image
WB analysis of various sample lysates using GTX55698 MCL1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
There are currently no references for MCL1 antibody (GTX55698). Be the first to share your publications with this product.
REVIEW
MCL1 antibody
Cat. No. GTX55698
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Chen-I Hsu submitted on 10-Sep-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
RD (ATCC CCL-136)
Amount used :
60μg
Blocking :
5% milk in TBST, 25°C, 16Hr
Primary Antibody :
1:2000, 4°C, 16Hr
Lane Description :
Lane1 : RD expressing shRNA targeting Luciferase
Lane2 : RD expressing shRNA targeting Mcl1 (TRCN0000005514)
Lane3 : RD expressing shRNA targeting Mcl1 (TRCN0000005515)
Lane4 : RD expressing shRNA targeting Mcl1 (TRCN0000005516)
Lane5 : RD expressing shRNA targeting Mcl1 (TRCN0000194663)
