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MCM2 antibody [1E7]

Anti-MCM2 antibody [1E7] used in Flow cytometry (FACS). GTX60520
Anti-MCM2 antibody [1E7] used in ELISA (ELISA). GTX60520
Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520
Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520
Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520
Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520
Anti-MCM2 antibody [1E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60520

Cat No. GTX60520

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($379)
See all MCM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MCM2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

minichromosome maintenance complex component 2 , BM28 , CCNL1 , CDCL1 , D3S3194 , DFNA70 , MITOTIN , cdc19

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Database

Research Area

DATA IMAGES

Anti-MCM2 antibody [1E7] used in Flow cytometry (FACS). GTX60520

GTX60520 FACS Image

FACS analysis of Jurkat cells using GTX60520 MCM2 antibody [1E7].
Green : MCM2
Red : negative control

Anti-MCM2 antibody [1E7] used in ELISA (ELISA). GTX60520

GTX60520 ELISA Image

ELISA analysis of antigen using GTX60520 MCM2 antibody [1E7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520

GTX60520 WB Image

WB analysis of human MCM2 (AA: 16-232) recombinant protein using GTX60520 MCM2 antibody [1E7].

Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520

GTX60520 WB Image

WB analysis of MCF-7 (1), HeLa (2), Jurkat (3), K562 (4), HEK293 (5) and HepG2 (6) cell lysate using GTX60520 MCM2 antibody [1E7].

Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520

GTX60520 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60520 MCM2 antibody [1E7].

Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520

GTX60520 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX60520 MCM2 antibody [1E7].

Anti-MCM2 antibody [1E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60520

GTX60520 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60520 MCM2 antibody [1E7].
Green : MCM2
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379