APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human MCM2 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
minichromosome maintenance complex component 2 , BM28 , CCNL1 , CDCL1 , D3S3194 , DFNA70 , MITOTIN , cdc19
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
Database
Research Area
DATA IMAGES
![Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520](/templates/default/js/lightbox/images/loading.gif "Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520")
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GTX60520 IHC-P Image
IHC-P analysis of ovarian cancer tissue using GTX60520 MCM2 antibody [1E7].
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GTX60520 IHC-P Image
IHC-P analysis of colon cancer tissue using GTX60520 MCM2 antibody [1E7].
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GTX60520 FACS Image
FACS analysis of Jurkat cells using GTX60520 MCM2 antibody [1E7].
Green : MCM2
Red : negative control
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GTX60520 ELISA Image
ELISA analysis of antigen using GTX60520 MCM2 antibody [1E7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60520 WB Image
WB analysis of MCF-7 (1), HeLa (2), Jurkat (3), K562 (4), HEK293 (5) and HepG2 (6) cell lysate using GTX60520 MCM2 antibody [1E7].
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GTX60520 WB Image
WB analysis of human MCM2 (AA: 16-232) recombinant protein using GTX60520 MCM2 antibody [1E7].
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GTX60520 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60520 MCM2 antibody [1E7].
Green : MCM2
Red: Actin filaments
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REFERENCE
There are currently no references for MCM2 antibody [1E7] (GTX60520). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCM2 antibody [1E7] (GTX60520). Be the first to share your experience with this product.
![Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520](/upload/website/prouct_img/small/GTX60520/GTX60520_20170912_IHC-P_1_w_23061123_716.webp "Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520")
![Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520](/upload/website/prouct_img/small/GTX60520/GTX60520_20170912_IHC-P_w_23061123_450.webp "Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520")
![Anti-MCM2 antibody [1E7] used in Flow cytometry (FACS). GTX60520](/upload/website/prouct_img/small/GTX60520/GTX60520_20170912_FACS_w_23061123_975.webp "Anti-MCM2 antibody [1E7] used in Flow cytometry (FACS). GTX60520")
![Anti-MCM2 antibody [1E7] used in ELISA (ELISA). GTX60520](/upload/website/prouct_img/small/GTX60520/GTX60520_20170912_ELISA_w_23061123_775.webp "Anti-MCM2 antibody [1E7] used in ELISA (ELISA). GTX60520")
![Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520](/upload/website/prouct_img/small/GTX60520/GTX60520_20170912_WB_1_w_23061123_926.webp "Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520")
![Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520](/upload/website/prouct_img/small/GTX60520/GTX60520_20170912_WB_w_23061123_776.webp "Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520")
![Anti-MCM2 antibody [1E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60520](/upload/website/prouct_img/small/GTX60520/GTX60520_20170912_ICCIF_w_23061123_666.webp "Anti-MCM2 antibody [1E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60520")
![Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520](/upload/website/prouct_img/normal/GTX60520/GTX60520_20170912_IHC-P_1_w_23061123_716.webp "Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520")
![Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520](/upload/website/prouct_img/normal/GTX60520/GTX60520_20170912_IHC-P_w_23061123_450.webp "Anti-MCM2 antibody [1E7] used in IHC (Paraffin sections) (IHC-P). GTX60520")
![Anti-MCM2 antibody [1E7] used in Flow cytometry (FACS). GTX60520](/upload/website/prouct_img/normal/GTX60520/GTX60520_20170912_FACS_w_23061123_975.webp "Anti-MCM2 antibody [1E7] used in Flow cytometry (FACS). GTX60520")
![Anti-MCM2 antibody [1E7] used in ELISA (ELISA). GTX60520](/upload/website/prouct_img/normal/GTX60520/GTX60520_20170912_ELISA_w_23061123_775.webp "Anti-MCM2 antibody [1E7] used in ELISA (ELISA). GTX60520")
![Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520](/upload/website/prouct_img/normal/GTX60520/GTX60520_20170912_WB_1_w_23061123_926.webp "Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520")
![Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520](/upload/website/prouct_img/normal/GTX60520/GTX60520_20170912_WB_w_23061123_776.webp "Anti-MCM2 antibody [1E7] used in Western Blot (WB). GTX60520")
![Anti-MCM2 antibody [1E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60520](/upload/website/prouct_img/normal/GTX60520/GTX60520_20170912_ICCIF_w_23061123_666.webp "Anti-MCM2 antibody [1E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60520")