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MCM2 antibody [CRCT2.1 (D1.9H5)]

Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] used in IHC (Paraffin sections) (IHC-P). GTX75459

Cat. No. GTX75459

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CRCT2.1 (D1.9H5)

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($399)
See all MCM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1/200

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Mcm2.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376816

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 2 , BM28 , CCNL1 , CDCL1 , D3S3194 , DFNA70 , MITOTIN , cdc19

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Database

Research Area

DATA IMAGES

Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] used in IHC (Paraffin sections) (IHC-P). GTX75459

GTX75459 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX75459 MCM2 antibody [CRCT2.1 (D1.9H5)].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399