GeneTex
United States (US)

MCM2 antibody [N1N2], N-term

IP/MS
GTX103071_40450_IP_19011506_470.jpg
GTX103071_40450_20160629_IFA_19011506_412.jpg
GTX103071_40450_WB_19011506_280.jpg

Cat No. GTX103071

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377385)

Predict Reactivity

Mouse, Rat, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BM28 antibody, CCNL1 antibody, CDCL1 antibody, D3S3194 antibody, KIAA0030 antibody, MGC10606 antibody, MITOTIN antibody, cdc19 antibody, MCM2 antibody, cell devision cycle-like 1 antibody, DNA replication licensing factor MCM2 antibody, nuclear protein BM28 antibody, minichromosome maintenance protein 2 homolog antibody, "MCM2 minichromosome maintenance deficient 2, mitotin antibody", minichromosome maintenance deficient 2 (mitotin) antibody, cyclin-like 1 antibody, minichromosome maintenance complex component 2 antibody

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103071_40450_IP_19011506_470.jpg

GTX103071 IP Image

MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 40 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term (GTX103071)
7.5 % SDS-PAGE
The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX103071_40450_20160629_IFA_19011506_412.jpg

GTX103071 ICC/IF Image

MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

GTX103071_40450_WB_19011506_280.jpg

GTX103071 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX103071 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169