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MCM2 antibody [N1N2], N-term

IP/MS
Anti-MCM2 antibody [N1N2], N-term used in Western Blot (WB). GTX103071
Anti-MCM2 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX103071
Anti-MCM2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103071

Cat. No. GTX103071

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MCM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

HCT116

Product Note

IP/MS validation was supported by references (PMID:30377385)

Predict Reactivity

Mouse, Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719639

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 2 , BM28 , CCNL1 , CDCL1 , D3S3194 , DFNA70 , MITOTIN , cdc19

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM2 antibody [N1N2], N-term used in Western Blot (WB). GTX103071

GTX103071 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX103071 diluted at 1:1000

Anti-MCM2 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX103071

GTX103071 IP Image

MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 40 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term (GTX103071)
7.5 % SDS-PAGE
The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-MCM2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103071

GTX103071 ICC/IF Image

MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Cheung CHY et al. Sci Rep 2017; 7 (1) : 13302 MCM2-regulated functional networks in lung cancer by multi-dimensional proteomic approach.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169