*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , HeLa , HepG2 , HCT116
IP/MS validation was supported by references (PMID:30377385)
Mouse, Rat, Chicken(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
minichromosome maintenance complex component 2 , BM28 , CCNL1 , CDCL1 , D3S3194 , DFNA70 , MITOTIN , cdc19
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq]
GTX103071 IP Image
MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 40 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term (GTX103071)
7.5 % SDS-PAGE
The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX103071 ICC/IF Image
MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
GTX103071 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX103071 diluted at 1:1000