APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HeLa , HepG2 , HCT116
Specificity/Sensitivity
IP/MS validation was supported by references (PMID:30377385)
Predict Reactivity
Mouse, Rat, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BM28 antibody, CCNL1 antibody, CDCL1 antibody, D3S3194 antibody, KIAA0030 antibody, MGC10606 antibody, MITOTIN antibody, cdc19 antibody, MCM2 antibody, cell devision cycle-like 1 antibody, DNA replication licensing factor MCM2 antibody, nuclear protein BM28 antibody, minichromosome maintenance protein 2 homolog antibody, "MCM2 minichromosome maintenance deficient 2, mitotin antibody", minichromosome maintenance deficient 2 (mitotin) antibody, cyclin-like 1 antibody, minichromosome maintenance complex component 2 antibody
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103071 IP Image
MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 40 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term (GTX103071)
7.5 % SDS-PAGE
The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX103071 ICC/IF Image
MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term (GTX103071) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
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GTX103071 WB Image
Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX103071 diluted at 1:1000
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REFERENCE
