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MCM2 antibody

Anti-MCM2 antibody used in Proximity ligation assay (PLA). GTX24461
Anti-MCM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX24461
Anti-MCM2 antibody used in Immunoprecipitation (IP). GTX24461

Cat. No. GTX24461

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, PLA

Reactivity

 Human, Xenopus
Package
50 μg ($399)
See all MCM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:10000
IHC-P 1:2000 - 1:10000
IP 2 - 5 μg/mg lysate
PLA 1:1000 - 1:10000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 37-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 2 encoded within exon 2 (LocusLink ID 4171).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 2 , BM28 , CCNL1 , CDCL1 , D3S3194 , DFNA70 , MITOTIN , cdc19

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM2 antibody used in Proximity ligation assay (PLA). GTX24461

GTX24461 PLA Image

PLA analysis of HeLa cells using GTX24461 MCM2 antibody and anti-human MCM6 antibody.
Red : positive signal
Blue : DAPI

Anti-MCM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX24461

GTX24461 IHC-P Image

IHC-P analysis of human small cell lung cancer tissue using GTX24461 MCM2 antibody.
Dilution : 1 μg/ml

Anti-MCM2 antibody used in Immunoprecipitation (IP). GTX24461

GTX24461 IP Image

Left : WB analysis of HeLa cell lysate (in RIPA buffer) using GTX24461 MCM2 antibody.
Loading : 50μg
Right : IP analysis of HeLa cell lysate using GTX24461 MCM2 antibody.
IP reaction : 20μg antibody/7mg lysate
Dilution : 0.2 mg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399