GeneTex
United States (US)

MCM2 antibody

GTX32716_20180530_IHC-P_18121410_720.jpg
GTX32716_20180530_WB_18121410_257.jpg

Cat No. GTX32716

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MCM2 (NP_004517.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Minichromosome Maintenance Complex Component 2,Bm28,Ccnl1,Cdcl1,D3S3194,Dfna70,Mitotin,Cdc19,Mcm2

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

Database

Research Area

DATA IMAGES
GTX32716_20180530_IHC-P_18121410_720.jpg

GTX32716 IHC-P Image

IHC-P analysis of human colon tissue using GTX32716 MCM2 antibody.
Dilution : 1:200

GTX32716_20180530_WB_18121410_257.jpg

GTX32716 WB Image

WB analysis of various samples using GTX32716 MCM2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329