Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Colon: A strong nuclear MCM3 immunostaining should be seen in virtually all crypt base cells.
Negative Control
Colon: MCM3 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human MCM3 fragment
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
minichromosome maintenance complex component 3 , HCC5 , P1-MCM3 , P1.h , RLFB
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
Database
Research Area
![Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424](/upload/website/prouct_img/small/GTX04424/GTX04424_20230728_IHC-P_73_23072722_307.webp "Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424")
![Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424](/upload/website/prouct_img/small/GTX04424/GTX04424_20230728_IHC-P_203_23072722_685.webp "Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424")
![Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424](/upload/website/prouct_img/small/GTX04424/GTX04424_20230728_IHC-P_333_23072723_614.webp "Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424")
![Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424](/upload/website/prouct_img/normal/GTX04424/GTX04424_20230728_IHC-P_73_23072722_307.webp "Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424")
![Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424](/upload/website/prouct_img/normal/GTX04424/GTX04424_20230728_IHC-P_203_23072722_685.webp "Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424")
![Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424](/upload/website/prouct_img/normal/GTX04424/GTX04424_20230728_IHC-P_333_23072723_614.webp "Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424")