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MCM3 antibody [MSVA-503M] HistoMAX™

HistoMAX
Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424
Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424
Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424

Cat. No. GTX04424

Host

Mouse

Clonality

Monoclonal

Clone Name

MSVA-503M

Isotype

IgG1

Application

IHC-P

Reactivity

Human
Package
500 μl ($569),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 7.8 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. Please refer to the protocol.

Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Colon: A strong nuclear MCM3 immunostaining should be seen in virtually all crypt base cells.

Negative Control

Colon: MCM3 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MCM3 fragment

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 3 , HCC5 , P1-MCM3 , P1.h , RLFB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]

Database

Research Area

DATA IMAGES

Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424

GTX04424 IHC-P Image

IHC-P analysis of human prostatic adenocarcinoma (PAC) tissue with (Gleason 3+3=6) tissue using GTX04424 MCM3 antibody [MSVA-503M] HistoMAX™.
Prostatic adenocarcinoma (Gleason 3+3=6) with strong MCM3 positivity of few tumor cells.

Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424

GTX04424 IHC-P Image

IHC-P analysis of human duodenum tissue using GTX04424 MCM3 antibody [MSVA-503M] HistoMAX™.
A strong MCM3 immunostaining occurs in duodenal crypt cells.

Anti-MCM3 antibody [MSVA-503M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04424

GTX04424 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX04424 MCM3 antibody [MSVA-503M] HistoMAX™.
Esophageal squamous epithelium with moderate to strong nuclear MCM3 staining of suprabasal and basal cells.

REFERENCE

There are currently no references for MCM3 antibody [MSVA-503M] HistoMAX™ (GTX04424). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MCM3 antibody [MSVA-503M] HistoMAX™ (GTX04424). Be the first to share your experience with this product.
Package List Price ($)
$ 569
$ 299