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MCM3 antibody [N1N3]

Anti-MCM3 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111160
Anti-MCM3 antibody [N1N3] used in Western Blot (WB). GTX111160
Anti-MCM3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111160
Anti-MCM3 antibody [N1N3] used in Western Blot (WB). GTX111160
Anti-MCM3 antibody [N1N3] used in Western Blot (WB). GTX111160

Cat. No. GTX111160

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MCM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCM3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950898

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 3 , HCC5 , P1-MCM3 , P1.h , RLFB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM3 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111160

GTX111160 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3(GTX111160) antibody at 1:500 dilution.

Anti-MCM3 antibody [N1N3] used in Western Blot (WB). GTX111160

GTX111160 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody [N1N3] (GTX111160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MCM3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111160

GTX111160 IHC-P Image

MCM3 antibody [N1N3] detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MCM3 antibody [N1N3] (GTX111160) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCM3 antibody [N1N3] used in Western Blot (WB). GTX111160

GTX111160 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX111160 diluted at 1:10000
Anti-MCM3 antibody [N1N3] used in Western Blot (WB). GTX111160

GTX111160 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody [N1N3] (GTX111160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169