GeneTex

  • Country / Location Selection

United States (US)

MCM3 antibody

IP/MS
Anti-MCM3 antibody used in Western Blot (WB). GTX110241
Anti-MCM3 antibody used in Western Blot (WB). GTX110241
Anti-MCM3 antibody used in Western Blot (WB). GTX110241
Anti-MCM3 antibody used in Immunoprecipitation (IP). GTX110241
Anti-MCM3 antibody used in ChIP assay (ChIP assay). GTX110241
Anti-MCM3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110241
Anti-MCM3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110241

Cat. No. GTX110241

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MCM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

HepG2

Product Note

IP/MS validation was supported by references (PMID:30377398)

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCM3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037393

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 3 , HCC5 , P1-MCM3 , P1.h , RLFB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM3 antibody used in Western Blot (WB). GTX110241

GTX110241 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody (GTX110241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MCM3 antibody used in Western Blot (WB). GTX110241

GTX110241 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX110241 diluted at 1:1000

Anti-MCM3 antibody used in Western Blot (WB). GTX110241

GTX110241 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody (GTX110241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MCM3 antibody used in Immunoprecipitation (IP). GTX110241

GTX110241 IP Image

MCM3 antibody immunoprecipitates MCM3 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 40 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM3 protein by 4 μg MCM3 antibody (GTX110241)
7.5 % SDS-PAGE
The immunoprecipitated MCM3 protein was detected by MCM3 antibody (GTX110241) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-MCM3 antibody used in ChIP assay (ChIP assay). GTX110241

GTX110241 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

Anti-MCM3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110241

GTX110241 ICC/IF Image

MCM3 antibody detects MCM3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM3 protein stained by MCM3 antibody (GTX110241) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-MCM3 antibody used in IHC (Paraffin sections) (IHC-P). GTX110241

GTX110241 IHC-P Image

MCM3 antibody detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MCM3 antibody (GTX110241) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Gao Y et al. Am J Cancer Res 2021; 11 (2) : 513-529 A heterogeneous cellular response to ionizing radiation revealed by single cell transcriptome sequencing.
Application : WB
Reactivity : Human
Essawy MM et al. Oral Dis 2020; Function of gold nanoparticles in oral cancer beyond drug delivery: Implications in cell apoptosis.
Application : IHC-P
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Guo L et al. Cell Death Differ 2012; 19 (12) : 1917-27 Histone demethylase Kdm4b functions as a co-factor of C/EBPβ to promote mitotic clonal expansion during differentiation of 3T3-L1 preadipocytes.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for MCM3 antibody (GTX110241). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169