MCM3 antibody

MCM3 antibody detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MCM3 antibody (GTX110241) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MCM3 antibody detects MCM3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM3 protein stained by MCM3 antibody (GTX110241) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

MCM3 antibody immunoprecipitates MCM3 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 40 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM3 protein by 4 μg MCM3 antibody (GTX110241)
7.5 % SDS-PAGE
The immunoprecipitated MCM3 protein was detected by MCM3 antibody (GTX110241) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody (GTX110241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX110241 diluted at 1:1000

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody (GTX110241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.