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MCM3 antibody

Anti-MCM3 antibody used in Western Blot (WB). GTX24460
Anti-MCM3 antibody used in IHC (Paraffin sections) (IHC-P). GTX24460
Anti-MCM3 antibody used in Proximity ligation assay (PLA). GTX24460
Anti-MCM3 antibody used in Western Blot (WB). GTX24460

Cat. No. GTX24460

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, PLA

Reactivity

 Human, Xenopus
Package
50 μl ($399)
See all MCM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000 - 1:10000
IHC-P 1:1000 - 1:5000
IP 2 - 5 μg/mg lysate
PLA 1:2000 - 1:40000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 38-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 10 and 11 (LocusLink ID 4172).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 3 , HCC5 , P1-MCM3 , P1.h , RLFB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM3 antibody used in Western Blot (WB). GTX24460

GTX24460 WB Image

WB analysis of HeLa cell lysate in RIPA buffer using GTX24460 MCM3 antibody.
Loading : 50μg
Dilution : 0.2 μg/ml

Anti-MCM3 antibody used in IHC (Paraffin sections) (IHC-P). GTX24460

GTX24460 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24460 MCM3 antibody.
Dilution : 1 μg/ml

Anti-MCM3 antibody used in Proximity ligation assay (PLA). GTX24460

GTX24460 PLA Image

PLA analysis of MCF-7 cells using GTX24460 MCM3 antibody and anti-human POLR2A antibody.
Green : Actin
Red : positive signal
Blue : DAPI

Anti-MCM3 antibody used in Western Blot (WB). GTX24460

GTX24460 WB Image

WB analysis of HeLa and 293T cell lysate prepared by NETN lysis buffer using GTX24460 MCM3 antibody.
Dilution : 0.1 μg/ml
Loading : 50μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399