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MCM3 antibody

Anti-MCM3 antibody used in Western Blot (WB). GTX55699
Anti-MCM3 antibody used in Immunoprecipitation (IP). GTX55699
Anti-MCM3 antibody used in IHC (Paraffin sections) (IHC-P). GTX55699
Anti-MCM3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55699

Cat. No. GTX55699

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all MCM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human MCM3 (NP_002379.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 3 , HCC5 , P1-MCM3 , P1.h , RLFB

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]

Database

Research Area

DATA IMAGES

Anti-MCM3 antibody used in Western Blot (WB). GTX55699

GTX55699 WB Image

WB analysis of various sample lysates using GTX55699 MCM3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-MCM3 antibody used in Immunoprecipitation (IP). GTX55699

GTX55699 IP Image

IP analysis of NIH/3T3 cell lysate using GTX55699 MCM3 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-MCM3 antibody used in IHC (Paraffin sections) (IHC-P). GTX55699

GTX55699 IHC-P Image

IHC-P analysis of human oophoroma tissue using GTX55699 MCM3 antibody.
Dilution : 1:100

Anti-MCM3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55699

GTX55699 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55699 MCM3 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399