APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Product Note
IP/MS validation was supported by references (PMID:30377397)
Predict Reactivity
Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950899
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
minichromosome maintenance complex component 4 , CDC21 , CDC54 , IMD54 , NKCD , NKGCD , P1-CDC21 , hCdc21
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109740 WB Image
MCM4 antibody detects MCM4 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE MCM4 antibody (GTX109740) dilution: 1:5000
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GTX109740 ChIP assay Image
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
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GTX109740 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody (GTX109740) diluted at 1:1000.
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GTX109740 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using MCM4(GTX109740) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109740 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM4(GTX109740) antibody at 1:500 dilution.
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GTX109740 IP Image
MCM4 antibody immunoprecipitates MCM4 protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM4 protein by 4 μg MCM4 antibody (GTX109740) 7.5 % SDS-PAGE The immunoprecipitated MCM4 protein was detected by MCM4 antibody (GTX109740) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX109740 WB Image
Sample (30 μg of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE GTX109740 diluted at 1:1000
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GTX109740 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using MCM4 antibody (GTX109740) at 1:300 dilution.
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REFERENCE
REVIEW
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