APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human MCM4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885284
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
minichromosome maintenance complex component 4 , CDC21 , CDC54 , IMD54 , NKCD , NKGCD , P1-CDC21 , hCdc21
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110424 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody (GTX110424) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 22354167 , PMID: 22354170
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GTX110424 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody (GTX110424) diluted at 1:2000.
|
|
|
GTX110424 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody (GTX110424) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 22354167 , PMID: 22354170
|
REFERENCE
There are currently no references for MCM4 antibody (GTX110424). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCM4 antibody (GTX110424). Be the first to share your experience with this product.
