GeneTex
United States (US)

MCM4 antibody

GTX32717_20180530_IHC-P_2_18121410_325.jpg
GTX32717_20180530_IHC-P_7_18121410_148.jpg
GTX32717_20180530_IHC-P_5_18121410_568.jpg
GTX32717_20180530_IHC-P_4_18121410_605.jpg
GTX32717_20180530_IHC-P_6_18121410_217.jpg
GTX32717_20180530_IHC-P_3_18121410_158.jpg
GTX32717_20180530_IHC-P_18121410_990.jpg
GTX32717_20180530_IHC-P_1_18121410_634.jpg
GTX32717_20180530_WB_18121410_980.jpg
GTX32717_20180530_IHC-P_8_18121410_776.jpg

Cat No. GTX32717

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

97 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide of human MCM4

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Minichromosome Maintenance Complex Component 4,Cdc21,Cdc54,Imd54,Nkcd,Nkgcd,P1-Cdc21,Hcdc21,Mcm4

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32717_20180530_IHC-P_2_18121410_325.jpg

GTX32717 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX32717 MCM4 antibody.
Dilution : 1:100

GTX32717_20180530_IHC-P_7_18121410_148.jpg

GTX32717 IHC-P Image

IHC-P analysis of rat pancreas tissue using GTX32717 MCM4 antibody.
Dilution : 1:100

GTX32717_20180530_IHC-P_5_18121410_568.jpg

GTX32717 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32717 MCM4 antibody.
Dilution : 1:200

GTX32717_20180530_IHC-P_4_18121410_605.jpg

GTX32717 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32717 MCM4 antibody.
Dilution : 1:100

GTX32717_20180530_IHC-P_6_18121410_217.jpg

GTX32717 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX32717 MCM4 antibody.
Dilution : 1:100

GTX32717_20180530_IHC-P_3_18121410_158.jpg

GTX32717 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX32717 MCM4 antibody.
Dilution : 1:200

GTX32717_20180530_IHC-P_18121410_990.jpg

GTX32717 IHC-P Image

IHC-P analysis of human B cell lymphoma tissue using GTX32717 MCM4 antibody.
Dilution : 1:200

GTX32717_20180530_IHC-P_1_18121410_634.jpg

GTX32717 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX32717 MCM4 antibody.
Dilution : 1:100

GTX32717_20180530_WB_18121410_980.jpg

GTX32717 WB Image

WB analysis of various samples using GTX32717 MCM4 antibody.
Dilution : 1:400
Loading : 25μg per lane

GTX32717_20180530_IHC-P_8_18121410_776.jpg

GTX32717 IHC-P Image

IHC-P analysis of rat pancreas tissue using GTX32717 MCM4 antibody.
Dilution : 1:200

Package List Price ($)
$ 329