GeneTex
  • Country / Location Selection

United States (US)

MCM4 (phospho Ser54) antibody

Anti-MCM4 (phospho Ser54) antibody used in Western Blot (WB). GTX32215

Cat No. GTX32215

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of MCM4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

minichromosome maintenance complex component 4 , CDC21 , CDC54 , IMD54 , NKCD , NKGCD , P1-CDC21 , hCdc21

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-MCM4 (phospho Ser54) antibody used in Western Blot (WB). GTX32215

GTX32215 WB Image

WB analysis of EGF-treated LO2 (A), HeLa (B), NIH3T3 (C), EGF-treated SP2/0 (D), EGF-treated H9C2 (E) whole cell lysates using GTX32215 MCM4 (phospho Ser54) antibody.

REFERENCE
There are currently no references for MCM4 (phospho Ser54) antibody (GTX32215). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCM4 (phospho Ser54) antibody (GTX32215). Be the first to share your experience with this product.
Package List Price ($)
$ 319