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MCM5 antibody

Anti-MCM5 antibody used in Western Blot (WB). GTX114090
Anti-MCM5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114090
Anti-MCM5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114090

Cat. No. GTX114090

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MCM5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCM5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620550

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 5 , CDC46 , MGORS8 , P1-CDC46

Cellular Localization

Nucleus

Background

The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM5 antibody used in Western Blot (WB). GTX114090

GTX114090 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
MCM5 antibody
GTX114090 diluted at 1:1000

Anti-MCM5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114090

GTX114090 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using MCM5(GTX114090) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCM5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114090

GTX114090 ICC/IF Image

MCM5 antibody detects MCM5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM5 protein stained by MCM5 antibody (GTX114090) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

Wang SJ et al. J Pers Med 2021; 11 (5) A Bioinformatics Analysis Identifies the Telomerase Inhibitor MST-312 for Treating High-STMN1-Expressing Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169