GeneTex
  • Country / Location Selection

United States (US)

MCM5 antibody

Anti-MCM5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114090
Anti-MCM5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114090
Anti-MCM5 antibody used in Western Blot (WB). GTX114090

Cat No. GTX114090

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCM5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620550

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

minichromosome maintenance complex component 5 , CDC46 , MGORS8 , P1-CDC46

Cellular Localization

Nucleus

Background

The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114090

GTX114090 ICC/IF Image

MCM5 antibody detects MCM5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM5 protein stained by MCM5 antibody (GTX114090) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-MCM5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114090

GTX114090 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using MCM5(GTX114090) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCM5 antibody used in Western Blot (WB). GTX114090

GTX114090 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
MCM5 antibody
GTX114090 diluted at 1:1000

REFERENCE

REVIEW

There are currently no reviews for MCM5 antibody (GTX114090). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169