MCM6 antibody

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM6 antibody (GTX129216) diluted at 1:1000.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM6 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM6 protein stained by MCM6 antibody (GTX129216) diluted at 1:500.
Blue: Hoechst 33342 staining.

MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
MCM6 antibody (GTX129216) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MCM6 antibody detects MCM6 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
MCM6 antibody (GTX129216) dilution: 1:1000

MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
MCM6 antibody (GTX129216) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MCM6 antibody immunoprecipitates MCM6 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of MCM6 protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of MCM6 by 3 μg of MCM6 antibody (GTX129216) 7.5% SDS-PAGE The immunoprecipitated MCM6 protein was detected by MCM6 antibody (GTX129216) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.