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MCM6 antibody

KO/KD IP/MS
Anti-MCM6 antibody used in Western Blot (WB). GTX129216
Anti-MCM6 antibody used in Immunoprecipitation (IP). GTX129216
Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129216
Anti-MCM6 antibody used in Western Blot (WB). GTX129216
Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129216
Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129216
Anti-MCM6 antibody used in ChIP assay (ChIP assay). GTX129216

Cat. No. GTX129216

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MCM6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Product Note

IP/MS validation was supported by references (PMID:30377417)

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MCM6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885924

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 6 , MCG40308 , Mis5 , P105MCM

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM6 antibody used in Western Blot (WB). GTX129216

GTX129216 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM6 antibody (GTX129216) diluted at 1:1000.

Anti-MCM6 antibody used in Immunoprecipitation (IP). GTX129216

GTX129216 IP Image

MCM6 antibody immunoprecipitates MCM6 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of MCM6 protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of MCM6 by 3 μg of MCM6 antibody (GTX129216) 7.5% SDS-PAGE The immunoprecipitated MCM6 protein was detected by MCM6 antibody (GTX129216) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129216

GTX129216 IHC-P Image

MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
MCM6 antibody (GTX129216) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCM6 antibody used in Western Blot (WB). GTX129216

GTX129216 WB Image

MCM6 antibody detects MCM6 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
MCM6 antibody (GTX129216) dilution: 1:1000

Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129216

GTX129216 IHC-P Image

MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
MCM6 antibody (GTX129216) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129216

GTX129216 ICC/IF Image

MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM6 protein stained by MCM6 antibody (GTX129216) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-MCM6 antibody used in ChIP assay (ChIP assay). GTX129216

GTX129216 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM6 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

MCM6 antibody Cat. No. GTX129216

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-MCM6 antibody used in Western Blot (WB). GTX129216
Date : Anonymous submitted on 16-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Amount used : 20 μg
Blocking : 4% milk, 25°C, 2Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169