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MCM6 antibody

Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129218
Anti-MCM6 antibody used in Western Blot (WB). GTX129218
Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129218
Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129218

Cat No. GTX129218

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MCM6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

minichromosome maintenance complex component 6 , MCG40308 , Mis5 , P105MCM

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129218

GTX129218 ICC/IF Image

MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MCM6 protein stained by MCM6 antibody (GTX129218) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-MCM6 antibody used in Western Blot (WB). GTX129218

GTX129218 WB Image

MCM6 antibody detects MCM6 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
MCM6 antibody (GTX129218) dilution: 1:1000

Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129218

GTX129218 IHC-P Image

MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
MCM6 antibody (GTX129218) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX129218

GTX129218 IHC-P Image

MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
MCM6 antibody (GTX129218) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169