GeneTex

  • Country / Location Selection

United States (US)

MCM6 antibody

Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54353
Anti-MCM6 antibody used in Immunoprecipitation (IP). GTX54353
Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54353
Anti-MCM6 antibody used in Western Blot (WB). GTX54353

Cat. No. GTX54353

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MCM6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 562-821 of human MCM6 (NP_005906.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 6 , MCG40308 , Mis5 , P105MCM

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-MCM6 antibody used in IHC (Paraffin sections) (IHC-P). GTX54353

GTX54353 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX54353 MCM6 antibody.
Dilution : 1:100

Anti-MCM6 antibody used in Immunoprecipitation (IP). GTX54353

GTX54353 IP Image

IP analysis of HeLa cell lysate using GTX54353 MCM6 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54353

GTX54353 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54353 MCM6 antibody.

Anti-MCM6 antibody used in Western Blot (WB). GTX54353

GTX54353 WB Image

WB analysis of various sample lysates using GTX54353 MCM6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for MCM6 antibody (GTX54353). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for MCM6 antibody (GTX54353). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399