MCM7 antibody [MCM7/1469]

Cat. No. GTX34820

Host	Mouse
Clonality	Monoclonal
Clone Name	MCM7/1469
Isotype	IgG2b
Application	WB, IHC-P, Protein Array
Reactivity	Human
Package	100 μg ($529)

See all MCM7 products

Datasheet
Document

View product citations for antibody GTX34820 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDC47 , MCM2 , MCM7 , P1.1MCM3 , P1CDC47 , P85MCM , PNAS146 , PPP1R104 , minichromosome maintenance complex component 7

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4176 MCM7
UniProt: P33993 MCM7

Research Area

DATA IMAGES

GTX34820 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34820 MCM7 antibody [MCM7/1469].

GTX34820 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Mouse Monoclonal Antibody (MCM7/1469). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

GTX34820 WB Image

WB analysis of MCF-7, HEK-293, A549, SKBr3, HepG2 lysates using GTX34820 MCM7 antibody [MCM7/1469].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty