MCM7 antibody [N2C2], Internal

Name: MCM7 antibody [N2C2], Internal
Brand: GeneTex
SKU: GTX110278
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-MCM7 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110278

Anti-MCM7 antibody [N2C2], Internal used in Western Blot (WB). GTX110278

Anti-MCM7 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110278

Anti-MCM7 antibody [N2C2], Internal used in ChIP assay (ChIP assay). GTX110278

Anti-MCM7 antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX110278

Cat. No. GTX110278

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all MCM7 products

View product citations for antibody GTX110278 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

H1299

Product Note

IP/MS validation was supported by references (PMID:30377399)

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MCM7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950901

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 7 , CDC47 , MCM2 , P1.1-MCM3 , P1CDC47 , P85MCM , PNAS146 , PPP1R104

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Gene ID: 4176 MCM7
UniProt: P33993 MCM7

Research Area

DATA IMAGES

GTX110278 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(GTX110278) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110278 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX110278 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110278 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM7(GTX110278) antibody at 1:200 dilution.

GTX110278 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

GTX110278 IP Image

MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal (GTX110278)
7.5 % SDS-PAGE
The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal (GTX110278) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.

Application : IP

Reactivity : Human

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REVIEW