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MCM7 antibody

Anti-MCM7 antibody used in Western Blot (WB). GTX00894
Anti-MCM7 antibody used in Immunoprecipitation (IP). GTX00894
Anti-MCM7 antibody used in Western Blot (WB). GTX00894
Anti-MCM7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00894

Cat No. GTX00894

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, ChIP assay

Reactivity

Human, Mouse, Rat, Hamster
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:200-1:1000
FACS Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified His6-tagged human MCM7 protein encompassing 562 -719 amino acids

Purification

Purified IgG
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

minichromosome maintenance complex component 7 , CDC47 , MCM2 , MCM7 , P1.1MCM3 , P1CDC47 , P85MCM , PNAS146 , PPP1R104

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MCM7 antibody used in Western Blot (WB). GTX00894

GTX00894 WB Image

WB analysis of various samples using GTX00894 MCM7 antibody. "*" : Indicates the band of MCM7 protein.
Lane 1 : untreated HeLa cells
Lane 2 : HeLa cells treated with 100 nM adriamycin for 24 hrs
Lane 3 : NIH-3T3 whole cell lysate
Dilution : 1:1000

Anti-MCM7 antibody used in Immunoprecipitation (IP). GTX00894

GTX00894 IP Image

IP analysis of WI38 whole cell lysate using GTX00894 MCM7 antibody. Cells were labeled with S³⁵ methionine and MCM7 was immunoprecipitated with the anti-MCM7 antibody followed by SDS-PAGE and autoradiography.
Lane 1 : Immunoprecipitation with pre-immune serum
Lane 2 : Immnoprecipitation with anti-MCM7 antiserum

Anti-MCM7 antibody used in Western Blot (WB). GTX00894

GTX00894 WB Image

WB analysis of various samples using GTX00894 MCM7 antibody.
Lane 1 : SiHa whole cell lysate
Lane 2 : C33A whole cell lysate
Lane 3 : WI38 whole cell lysate

Anti-MCM7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00894

GTX00894 ICC/IF Image

ICC/IF analysis of G1 phase HeLa cell nucleus using GTX00894 MCM7 antibody. The HeLa cells was treated with protein cross-linking reagent, DSP and chromatin extraction. Photographed with confocal microscope.
Fixation : 4% PFA

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 359