APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:5000 |
| 1:200-1:1000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified His6-tagged human MCM7 protein encompassing 562 -719 amino acids
Purification
Purified IgG
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
minichromosome maintenance complex component 7 , CDC47 , MCM2 , MCM7 , P1.1MCM3 , P1CDC47 , P85MCM , PNAS146 , PPP1R104
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00894 WB Image
WB analysis of various samples using GTX00894 MCM7 antibody. "*" : Indicates the band of MCM7 protein.
Lane 1 : untreated HeLa cells
Lane 2 : HeLa cells treated with 100 nM adriamycin for 24 hrs
Lane 3 : NIH-3T3 whole cell lysate
Dilution : 1:1000
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GTX00894 WB Image
WB analysis of various samples using GTX00894 MCM7 antibody.
Lane 1 : SiHa whole cell lysate
Lane 2 : C33A whole cell lysate
Lane 3 : WI38 whole cell lysate
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GTX00894 IP Image
IP analysis of WI38 whole cell lysate using GTX00894 MCM7 antibody. Cells were labeled with S³⁵ methionine and MCM7 was immunoprecipitated with the anti-MCM7 antibody followed by SDS-PAGE and autoradiography.
Lane 1 : Immunoprecipitation with pre-immune serum
Lane 2 : Immnoprecipitation with anti-MCM7 antiserum
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GTX00894 ICC/IF Image
ICC/IF analysis of G1 phase HeLa cell nucleus using GTX00894 MCM7 antibody. The HeLa cells was treated with protein cross-linking reagent, DSP and chromatin extraction. Photographed with confocal microscope.
Fixation : 4% PFA
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REFERENCE
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REVIEW
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