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MCM7 antibody

Anti-MCM7 antibody used in Immunoprecipitation (IP). GTX54354
Anti-MCM7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54354
Anti-MCM7 antibody used in Western Blot (WB). GTX54354

Cat. No. GTX54354

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all MCM7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 360-719 of human MCM7 (NP_005907.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

minichromosome maintenance complex component 7 , CDC47 , MCM2 , P1.1-MCM3 , P1CDC47 , P85MCM , PNAS146 , PPP1R104

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MCM7 antibody used in Immunoprecipitation (IP). GTX54354

GTX54354 IP Image

IP analysis of HeLa cell lysate using GTX54354 MCM7 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:500

Anti-MCM7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54354

GTX54354 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54354 MCM7 antibody.
Blue : DAPI
Dilution : 1:100

Anti-MCM7 antibody used in Western Blot (WB). GTX54354

GTX54354 WB Image

WB analysis of various sample lysates using GTX54354 MCM7 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399